Sirt1 Runx2 Signaling Pathway Promotes Healing of Tibial Fracture in Rats |
| You Lyu Li Fuli Liu Guiguang |
| Department of Orthopaedics, Beijing Beiya Orthopaedics Hospital, Beijing 102445, China |
Abstract : Objective: To investigate the effects of Sirtuin-1 (Sirt1)/Runt-related transcription factor 2 (Runx2) signaling pathway on the healing of tibial fracture in rats. Methods: Rat models of tibial fracture were established and hen divide d into fracture group (Fra group) and resveratrol treatment group (RES group). Next, the healing of fracture was exami ned by X-ray inspection. Bone marrow mesenchymal stem cells (BMSCs) were isolated from rats and cultured in vitro. Results: Rat models of tibial fracture were successfully established, and BMSCs were isolated and cultured.RES pro moted the healing of fracture in rats and the gene expressions of Sirt1 and Runx2 at fracture ends and in osteobla sts induced in vitro. According to alizarin red staining assay, RES facilitated the osteogenic differentiation of BMSCs , while EX-527 inhibited their osteogenic differentiation. QRT-PCR results revealed that the messenger ribonuc leic acid (mRNA) expressions of Sirt1 and Runx2 at tibial fractures at 24 h after fracture were significantly higher than th ose in sham group, and they were the highest in RES group. In addition, the mRNA expression levels of Sirt1 and Runx 2 were overtly higher in induction group and RES co-induction group than those in control group, whereas the indu ction with EX 527 evidently inhibited the mRNA expressions of Sirt1 and Runx2 at 2 w and 3 w. At 3 w after oste ogenic induction of BMSCs, both induction group and RES co-induction group had significantly increased protein exp ression levels of Sirt1 and Runx2. Conclusions: The Sirt1/Runx2 signaling pathway facilitates the healing of tibial fra cture in rats.
Keywords BMSCs;Sirt1/Runx2 signaling pathway;rats;tibial fracture
|
|
|
|
|
Document Retrieval Certificate / Proof of Publication Indexing
Author Contribution Statement / Certificate of Authorship Contribution
Peer Review Report / Peer Review Comments
Conflict of Interest
The author declares no conflict of interest.
|
|
